Benjamin U Ebeshi, Edebi N Vaikosen, Kemetimiware T Temes
Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria;For correspondence:- Benjamin Ebeshi Email:
Published: 31 December 2015
Citation: Ebeshi BU, Vaikosen EN, Temes KT. High performance thin layer chromatographic and ultra-violet spectrophotometric fingerprinting of some beta lactam antibiotics. J Sci Pract Pharm 2015; 2(1):64-69 doi: 10.47227/jsppharm.v2i1.13
© 2015 The author(s).
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Purpose: This work describes thin layer chromatographic (TLC) analysis and spectrophotometric fingerprinting procedures that allow easy and rapid separation and identification of beta-lactam antibiotics (penicillins and cephalosporins) from complex mixtures.
Methods: Using silica gel GF254 as the stationary phase and choosing different mobile phases we successfully developed a cost effective and efficient analytical method for the separation and identification of the studied beta-lactam antibiotics. Also using a UV spectrophotometer, beta-lactam antibiotics were identified through evaluation of the absorbance they produce at different wavelengths (from 250nm to 430nm).
Results: Beta-lactam antibiotics can be detected in UV light at 254nm and 366nm (producing colours / fluorescence) and detection limit can be reduced with the help of colour reactions (especially reaction with iodine under iodine vapour). Spectrophotometric fingerprinting analysis of beta lactam antibiotics, where maximum absorption is obtained at a wavelength of 350nm after an initial lower absorbance reading at 340nm as well as a reduction in absorbance to zero or negative value at 430nm.
Conclusion: Appropriate solvent system and method of detection permits the identification of the entire beta-lactam antibiotics studied. TLC analysis and spectrophotometric fingerprinting procedures can be successfully applied in preparatory and exploratory analytical screening, quality control studies, therapeutic drug monitoring of beta-lactam antibiotics.
